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03-19-2017, 10:16 PM
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#1 |
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Junior Member
Location: Israel Join Date: Mar 2017
Posts: 1
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PhiX control for a custom made library
I am preparing a custom made PCR-based amplicon library.
I will use custom primers for the read in miseq but since it is a low diversity library, I need some control as PhiX. However, PhiX works only with illuminas primers and will not work with my custom made primers. What can I use instead of PhiX? Do you there is an option to add my custom primers to Illumina's read1 and read 2 standard primers and by this keep using the PhiX control? thank you |
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03-20-2017, 03:05 AM
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#2 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 6,700
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That should be no problem. See this thread for more: http://seqanswers.com/forums/showthread.php?t=39717
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