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Old 03-19-2017, 10:16 PM   #1
ymym
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Location: Israel

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Default PhiX control for a custom made library

I am preparing a custom made PCR-based amplicon library.
I will use custom primers for the read in miseq but since it is a low diversity library, I need some control as PhiX. However, PhiX works only with illuminas primers and will not work with my custom made primers.
What can I use instead of PhiX?
Do you there is an option to add my custom primers to Illumina's read1 and read 2 standard primers and by this keep using the PhiX control?

thank you
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Old 03-20-2017, 03:05 AM   #2
GenoMax
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Default

That should be no problem. See this thread for more: http://seqanswers.com/forums/showthread.php?t=39717
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