Hi all,
I am working about denovo assembly of non-model organism by Trinity assembler but i still confusing of definition's "singleton".
Definition 1: Some papers and journals define singleton: TGICL cluster contig(transcripts) from Trinity's output and we have 2 class is "Cluster" and "Singleton". (http://www.plosone.org/article/info%...l.pone.0014233)
Definition 2: But when i read some website or blog i realize singleton is reads which do not overlap with other reads in assembly process
So which 's definition right? and if "definition 2" 's right, how to extract "non-overlap reads" from Trinity's output (transcripts)
Thanks in advance.
I am working about denovo assembly of non-model organism by Trinity assembler but i still confusing of definition's "singleton".
Definition 1: Some papers and journals define singleton: TGICL cluster contig(transcripts) from Trinity's output and we have 2 class is "Cluster" and "Singleton". (http://www.plosone.org/article/info%...l.pone.0014233)
Definition 2: But when i read some website or blog i realize singleton is reads which do not overlap with other reads in assembly process
So which 's definition right? and if "definition 2" 's right, how to extract "non-overlap reads" from Trinity's output (transcripts)
Thanks in advance.
Comment