I set a my own reference database containg selected genes (n=1500).
I had a 454 data about 100MB.
I run Bowtie 2 to get SAM, sorted/indexed. How do I get a excel-like raw read count output to each mapped reference gene? I mean simple count rather than expression study.
I noted there are some suggestion in the forum to use Bedtools or HTseq. Are they appropriate for solving my issue. If so, which performs better?
Thanks a lot.
I had a 454 data about 100MB.
I run Bowtie 2 to get SAM, sorted/indexed. How do I get a excel-like raw read count output to each mapped reference gene? I mean simple count rather than expression study.
I noted there are some suggestion in the forum to use Bedtools or HTseq. Are they appropriate for solving my issue. If so, which performs better?
Thanks a lot.
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