Dear members,
Following my 454 data assembly, sequenced from a cDNA library, I'm looking for an "automated way" to find ORF for all the consensus contig sequences. My assembly was performed with a high percentage of similarity (0.98) using CLC workbench. Unfortunately, in my favorite species, there is no complete genome available at this moment to gather any information.
So, did anyone have already experienced this problem? How to find the true ORF with 454 contig that may contains some errors, such as false-positive insertion that shift the frame?
Thanks a lot,
Regards,
Christopher
Following my 454 data assembly, sequenced from a cDNA library, I'm looking for an "automated way" to find ORF for all the consensus contig sequences. My assembly was performed with a high percentage of similarity (0.98) using CLC workbench. Unfortunately, in my favorite species, there is no complete genome available at this moment to gather any information.
So, did anyone have already experienced this problem? How to find the true ORF with 454 contig that may contains some errors, such as false-positive insertion that shift the frame?
Thanks a lot,
Regards,
Christopher
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