Hi,
I'm new in sequencing, next week I'm going to prepare a small RNA library using NEBNExt Multiplex small RNA prep kit for Illumina from New England biolabs.
I'm watching some tutorials from Illumina and I'm stumbling on this:
Workflow: small RNA
What's It's Used For: Sequencing of cDNA following reverse transcription of small RNA. Annotation backed by mirBase and Rfam. Requires contaminants, RNA, and miRNA FASTA files, in addition to the reference genome. (Human genome is included on MiSes.) FASTQ files and stats files are available for subsequent downstream analysis.
I don't see any requirements for all the other workflows, why does this workflow need one? And where do I get that information?
I'm new in sequencing, next week I'm going to prepare a small RNA library using NEBNExt Multiplex small RNA prep kit for Illumina from New England biolabs.
I'm watching some tutorials from Illumina and I'm stumbling on this:
Workflow: small RNA
What's It's Used For: Sequencing of cDNA following reverse transcription of small RNA. Annotation backed by mirBase and Rfam. Requires contaminants, RNA, and miRNA FASTA files, in addition to the reference genome. (Human genome is included on MiSes.) FASTQ files and stats files are available for subsequent downstream analysis.
I don't see any requirements for all the other workflows, why does this workflow need one? And where do I get that information?
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