I'm trying to create a reference genome with BFAST using the inhouse reference genome. This file contains all the chromosomes of the human genome and is ~3 GB large. However, when I execute the following command:
bfast fasta2brg -f /../reference_human.fa -A 0
It gives the following error:
************************************************************
Checking input parameters supplied by the user ...
Validating fastaFileName /../reference_human.fa.
Input arguments look good!
************************************************************
************************************************************
Printing Program Parameters:
programMode: [ExecuteProgram]
fastaFileName: /../reference_human.fa
space: [NT Space]
timing: [Not Using]
************************************************************
************************************************************
Reading from /../reference_human.fa.
Reading in [contig,pos]:
]-------1,----------0]Base:[
************************************************************
In function "RGBinaryRead": Fatal Error[OutOfRange]. Variable/Value: original.
Message: Not a valid base pair.
***** Exiting due to errors *****
************************************************************
When I run the chromosomes seperately the tool functiones fine. I can't find the source of the problem. What is going wrong here?
Is it possible to execute the run using multiple reference files? (Since the tool seems to work with a chromosome at a time)
bfast fasta2brg -f /../reference_human.fa -A 0
It gives the following error:
************************************************************
Checking input parameters supplied by the user ...
Validating fastaFileName /../reference_human.fa.
Input arguments look good!
************************************************************
************************************************************
Printing Program Parameters:
programMode: [ExecuteProgram]
fastaFileName: /../reference_human.fa
space: [NT Space]
timing: [Not Using]
************************************************************
************************************************************
Reading from /../reference_human.fa.
Reading in [contig,pos]:
]-------1,----------0]Base:[
************************************************************
In function "RGBinaryRead": Fatal Error[OutOfRange]. Variable/Value: original.
Message: Not a valid base pair.
***** Exiting due to errors *****
************************************************************
When I run the chromosomes seperately the tool functiones fine. I can't find the source of the problem. What is going wrong here?
Is it possible to execute the run using multiple reference files? (Since the tool seems to work with a chromosome at a time)
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