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Old 04-18-2013, 01:15 PM   #1
Location: Cambridge

Join Date: Apr 2013
Posts: 32
Default CASIM: RNA-Seq Factors in experimental design

Which of these factors is important in RNA-Seq analysis
  • global vs nuclear RNA
  • strand-specific vs not
  • mapped to known transcripts only vs de novo transcript calls
  • alternative splicing considered vs not
  • 50 bp vs 100+ bp, single end vs paired end
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