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Most researchers listed improved quantitation as one of the most critical issues with RNA-seq. Bioo Scientific has listened and launched the patent pending NEXTflex™ Rapid Directional qRNA-Seq™ Kit which incorporates Molecular Indexes™ to allow researchers to easily distinguish amplified copies of their transcripts from actual duplicated transcripts found in the cell. The NEXTflex Rapid Directional qRNA-Seq Kit utilizes a dUTP-based protocol to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from.
For more information read our white paper written in collaboration with Cellular Research, which demonstrates how incorporating Molecular Indexes can improve the quantitation of transcriptome analysis.
More information about using Molecular Indexes™ to improve RNA-Seq quantitation can also be read in this recent publication:
Fu, G.K., Xu, W., Wilhelmy, J., Mindrinos, M.N., Davis, R.W., Xiao, W., and Fodor, S.P.A. 2013. Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations. PNAS. doi: 10.1073/pnas.1323732111
For more information read our white paper written in collaboration with Cellular Research, which demonstrates how incorporating Molecular Indexes can improve the quantitation of transcriptome analysis.
More information about using Molecular Indexes™ to improve RNA-Seq quantitation can also be read in this recent publication:
Fu, G.K., Xu, W., Wilhelmy, J., Mindrinos, M.N., Davis, R.W., Xiao, W., and Fodor, S.P.A. 2013. Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations. PNAS. doi: 10.1073/pnas.1323732111