Shipping RNA samples with RNAstable for RNA sequencing

[machado]

I want to send several RNA samples to China for sequence the transcriptome using the Illumina HiSeq 2000 technology.
However, the dry ice shipping costs are too high. To solve this problem, we find a new way to transport RNA that doesn’t need dry ice (the transportation could be done at room temperatures), using RNAstable technology from Biomatrica company.
Still, I’m afraid that RNAstable matrix could impair the Illumina RNA sequencing technology.

Therefore, I’d like to ask if anyone knows if RNAstable matrix will allow the transcriptome sequencing using Illumina technology without any problem.

Thank you for your time

Best regards

Miguel Machado

[machado]

Good News

Dear all

We’ve been using RNAstable to ship RNA samples from Portugal to China for RNA-Seq by Illumina technology, and everything was fine (shipment, library construction and sequencing process). We’ve done it twice.
Now we are arranging the things to ship RNA for Solid sequencing in RNAstable to the heart of Amazonic forest (INPA, Manaus, Brazil).
As someone told me some time ago, RNA shipment in dry ice is something to forget.

Best regards

Miguel Machado

[chipmonk]

what samples are these

dear machado
I also wish to ship but what tissue samples are these. i have a tissue which is jelly like and size of a drosophila embryo. do you think this can be shipped in rnalater kind of reagent. will penetrance be an issue. please give your opinion. thanks
regards
chipmonk

[machado]

dear machado
I also wish to ship but what tissue samples are these. i have a tissue which is jelly like and size of a drosophila embryo. do you think this can be shipped in rnalater kind of reagent. will penetrance be an issue. please give your opinion. thanks
regards
chipmonk

Hi chipmonk
The RNAstable system is suitable for long storing extracted RNA, not RNA in tissues.
For preserving RNA in tissues you can use RNA later from Ambion or Qiagen, but this system has time limitations, like 1 day at 37 ºC.
I hope this was useful.
Thanks
Machado