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  • New! Encore Complete RNA-Seq Library Systems from NuGEN Technologies

    The Encore Complete RNA-Seq Library Systems offer a number of advantages for researchers engaged in RNA-Seq studies:

    An end-to-end solution for strand-specific RNA-Seq – All required components for low input preparation of strand-specific RNA-Seq libraries from moderate amounts of total RNA without the need for rRNA reduction or poly(A) selection
    Superior strand retention – Using a range of sources for total RNA researchers can expect to achieve >95% read mapping in the sense orientation for accurate and complete understanding of transcript profiling results
    Affordable and scalable kit configurations – The barcoding capability provided with Encore Complete RNA-Seq Multiplex Systems I and IB improve sample throughput and reduce sequencing costs as your projects grow



    Best,
    Steve
    Last edited by NuGEN; 10-21-2011, 11:57 AM. Reason: Adding link to product web page

  • #2
    Hi Steve,

    We recently sequenced Nugen ENCORE complete RNA seq library samples on our HiSeq 2000. The q30 scores were not great (between 70-80% - we normally see q30s in the 90s). The phasing was high on some of the lanes and the prephasing was high on other lanes. We also happened to run 3 Illumina prepped samples that had normal q30s and normal phasing/prephasing. Have you heard of issues like this before? Do you know why the NuGen prepped samples would behave this way?

    Thanks,
    Jeanne
    Last edited by seq2no; 02-13-2012, 07:01 AM. Reason: spelling error

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