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  • Single-cell analysis: from DNA to RNA world

    Hi there,

    I'm new to this community, so this is to say hello and - at the same time - thanks to those providing helpful hints and information...

    I was doing single/rare-cell DNA analysis and joined a team working on single-cell RNA issues...
    ... now that I got introduced to the RNA world (which caused some extra adaptation in the lab as my DNA-lab-reference-system is kind of resisting to hand over to RNA) I more or less feel comfortable and can dig into things.

    The reason for being here is that I'm working on single-cell RNA sequencing which is why I started to follow the Smart-Seq2 protocol published earlier this year. Happy about the info I already got by surfing through the posts I might come up with one or two myself...

    Looking forward to interact ...

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  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

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