Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Whole genome alignment (or the opposite of it?) jomaco Bioinformatics 1 02-14-2013 05:57 AM
Bowtie giving opposite results rcook34 Bioinformatics 1 11-29-2012 01:30 PM
What is a Probe Set? What is a Probe pair? Pixel08 General 1 12-31-2011 02:15 PM
Adding nucleotides onto the 5' end of adapter uberfinch Illumina/Solexa 0 12-06-2011 02:57 PM

Thread Tools
Old 07-31-2017, 08:08 AM   #1
Junior Member
Location: New Jersey

Join Date: Jul 2017
Posts: 2
Default Why adding adapter first and probe later, but not the opposite?

I saw it is common to add adapter first and then use probe to grep interested fragments in lots of NGS protocol.

I am wonder why don't we do the opposite? Would't that be more efficient since that way we can only target fragments being bind with probes and don't have to waste adapters on unwanted fragments? Is there any technical reason that make the protocol the way it is? Any comments are appreciate!
UniCornal is offline   Reply With Quote
Old 07-31-2017, 08:23 AM   #2
Senior Member
Location: Bethesda MD

Join Date: Oct 2009
Posts: 504

Adapter ligation is a concentration-dependent reaction. If enrichment is performed first, then the amount of one of the reactants (fragments) becomes low, which reduces the efficiency of the reaction (i.e., less library).

P.S. - It's not necessary to post the same question in multiple topic areas.
HESmith is offline   Reply With Quote

ngs, sequencing

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:42 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO