i'm very new to RNA-seq and I'm trying to extract PBMC for RNA sequencing.
I know that there is the ficoll technique to isolate PBMC using different density gradient centrifugation method, but this technique can is too long for me to extract sufficient RNA and the process might alter the RNA that might not be reflective of the condition.
Is there anyone out there that has another recommendation?
is it more suitable if i extract rna from whole blood since there is no DNA in red blood cells and they cannot synthesise rna.
I know that there is the ficoll technique to isolate PBMC using different density gradient centrifugation method, but this technique can is too long for me to extract sufficient RNA and the process might alter the RNA that might not be reflective of the condition.
Is there anyone out there that has another recommendation?
is it more suitable if i extract rna from whole blood since there is no DNA in red blood cells and they cannot synthesise rna.
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