Hi Everyone,
I am trying to de novo assemble a draft bacterial genome with a single chromosome from another persons data. The insert size of the sequenced segments average to 580bp and the read length for the R1 and R2 files is 301. After filtering there is not enough overlap to merge the paired reads. Is there a way to still use this data to assemble a draft genome that can be annotated?
I am trying to de novo assemble a draft bacterial genome with a single chromosome from another persons data. The insert size of the sequenced segments average to 580bp and the read length for the R1 and R2 files is 301. After filtering there is not enough overlap to merge the paired reads. Is there a way to still use this data to assemble a draft genome that can be annotated?
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