Hi all,
When cleaning up a library (Nextera generated if that is important), how common an observation is it to see bias towards recovery of smaller DNA fragments when using SPRI beads - either Axygen magPCR cleanup OR AMPureXP?
We generate fragments in the range of 150 - 800 for our workflow, but when we clean-up using the beads the distribution skews somewhat to the left towards the smaller fragments (150 - 250).
If this is common, is there a way to minimise the effect? Or can anyone offer me any thoughts on the cause and/or solution?
Many thanks!
When cleaning up a library (Nextera generated if that is important), how common an observation is it to see bias towards recovery of smaller DNA fragments when using SPRI beads - either Axygen magPCR cleanup OR AMPureXP?
We generate fragments in the range of 150 - 800 for our workflow, but when we clean-up using the beads the distribution skews somewhat to the left towards the smaller fragments (150 - 250).
If this is common, is there a way to minimise the effect? Or can anyone offer me any thoughts on the cause and/or solution?
Many thanks!
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