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    Hello All,
    NGS data from SRA is downloaded in form of .sra files which are highly compressed and save a lot of space. fastq-dump converts .sra to fastq. i am interested in something which converts fastq, back to sra, i learnt fastq-load does it, but cannot find any manual explaining how to. Does anyone know how to and can help?

  • #2
    well, running the help from the command line will probably give you quite a lot of help:
    Code:
    $ fastq-load --help
    
    Usage:
            fastq-load [options] -r run.xml -e experiment.xml -o output-path
    
      -r|--run-xml                     path to run.xml describing input files 
      -e|--experiment                  path to experiment.xml 
      -o|--output-path                 target location 
    
    Options:
      -i|--input-path                  input files location, default '.' 
      -u|--input-unpacked              input files are unpacked 
    ... etc.
    The XML schemas for Run / Experiment can be found on the NCBI SRA website.

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