I am doing targeted RNA-seq using specific primers against known fusion partners (e.g., primer against BCR and primer against ABL1). I am seeing some non-targeted fusions, i.e., fusions which I am not expecting in a given sample. Some of these fusions are seen after repeat library prep and re-sequencing but most do not repeat. I am trying to think about how to interpret these - whether they are artifacts or biologically present at a low level. Does anyone have any references or approaches that they think are helpful for this?
Thanks,
seq-ngs
Thanks,
seq-ngs
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