Hello friends
I am getting trouble trying to analyze some BLAST results executing Megan 5 via command line. When I use the "import Blast" option of the GUI, I've got no problems reading the file and getting results. But when trying to do the same using the command line, I cannot get anything. Here is my command:
~/megan/MEGAN -g -x "load taxGIFile=gi_taxid_prot.bin; import blastfile=./my.blastx meganfile=test.blastx.rma"
Result: no hits are recognized (trece below). Even if it recognizes correctly the blasttab format of the file. Any hint on this, please? Thanks a lot!
Input format: BlastTAB
Processing my.blastx
Processing BlastTAB file(s)
Note: Reads file(s) not given or found, RMA file will not contain read sequences.
Total reads: 161001
Total no-hits: 161001
Total matches: 8360785
Matches discarded: 5234055
Parsing required 118 seconds
Running Data analyzer: Init
Analyzing all matches
Applying min-support filter
Number of changes due to min-support filter: 0
Number of reads: 80501
Low complexity: 0
With valid hits: 0
MEGAN> Total reads: 80501
Assigned reads: 0
Unassigned reads: 80501
Reads with no hits: 0
Reads low comp.: 0
Induce Taxonomy tree, keeping 2 of 1111248 nodes
I am getting trouble trying to analyze some BLAST results executing Megan 5 via command line. When I use the "import Blast" option of the GUI, I've got no problems reading the file and getting results. But when trying to do the same using the command line, I cannot get anything. Here is my command:
~/megan/MEGAN -g -x "load taxGIFile=gi_taxid_prot.bin; import blastfile=./my.blastx meganfile=test.blastx.rma"
Result: no hits are recognized (trece below). Even if it recognizes correctly the blasttab format of the file. Any hint on this, please? Thanks a lot!
Input format: BlastTAB
Processing my.blastx
Processing BlastTAB file(s)
Note: Reads file(s) not given or found, RMA file will not contain read sequences.
Total reads: 161001
Total no-hits: 161001
Total matches: 8360785
Matches discarded: 5234055
Parsing required 118 seconds
Running Data analyzer: Init
Analyzing all matches
Applying min-support filter
Number of changes due to min-support filter: 0
Number of reads: 80501
Low complexity: 0
With valid hits: 0
MEGAN> Total reads: 80501
Assigned reads: 0
Unassigned reads: 80501
Reads with no hits: 0
Reads low comp.: 0
Induce Taxonomy tree, keeping 2 of 1111248 nodes
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