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  • annotations intervals problem

    Hi guys,

    I have two whole genome sequence pf bacteria, mutant and wild type.

    I got this annotates in RAST server. and when i try to compare any specific gene and gene product....there is some variation.

    for example
    In mutant type:
    Name: ABC transporter permease protein CDS
    Type: CDS
    Total Length: 2517 (over 16 intervals)
    Interval 16 Length: 501
    Interval 16: 1,919,937 -> 1,920,437
    Interval 16 Bases: ATCAATCGCGTCAGTCGTAAG...
    Intervals: ...1,919,852 -> 1,919,893, 1,919,897 -> 1,919,935, 1,919,937 -> 1,920,437
    Bases: GTGAGCAAAATGCGTAAAACA......
    NCBI Join Type: join
    db_xref: GO:0005215
    db_xref: GO:0006810
    db_xref: GO:0016020
    translation: MSKMRKTILWKDAFQAITHSLGRYIAIILLIGLGTFAFTGLKMAGPDMRATGADFFNKHNLADVTITSNCGINSTDRMTIRNLSEVKKQRLAIFKxx...
    product: ABC transporter permease protein
    NCBI Feature Key: CDS


    In wild type:
    Name: ABC transporter permease protein CDS
    Type: CDS
    Length: 2541
    Interval: 1,925,619 -> 1,928,159
    Bases: ATGCGTAAAACAATTCTATGG...
    db_xref: GO:0005215
    db_xref: GO:0006810
    db_xref: GO:0016020
    translation: MRKTILWKDAFQAITHSLGRYIAIILLIGLGTFAFTGLKMAGPDMRATGADFFNKHNLADVTITSNCGINSTDRMTIRNLSEVKKATFGYFQDVKNK...
    product: ABC transporter permease protein
    NCBI Feature Key: CDS



    can anyone tell me why it shows the gene info as intervals..its happening in many of the CDS....can anyone help me...

  • #2
    Also one more question....

    However, However, i have a query about annotation. I have two WGS, one mutant and other wild type.....
    while performing the annotation, if there is any mutation in the promoter region, will that be disregarded for annotation??......could u please breif me through it..

    i use RAST server for annotation purposes....

    could somebody help me...please

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