Hello
I am designing the exome sequencing of breast cancer tissues.
The tissues would be FFPE (Formalin Fixed Paraffin Embedded).
I wonder what level of coverage would be good for rare SNP or structural variations in exome sequencing.
I was thinking 40X, however, I found a few papers using 100X~200X exome sequencing.
Plus, any specific caution for FFPE tissues which are likely to include degraded DNA ?
Please help me !
Thank you
I am designing the exome sequencing of breast cancer tissues.
The tissues would be FFPE (Formalin Fixed Paraffin Embedded).
I wonder what level of coverage would be good for rare SNP or structural variations in exome sequencing.
I was thinking 40X, however, I found a few papers using 100X~200X exome sequencing.
Plus, any specific caution for FFPE tissues which are likely to include degraded DNA ?
Please help me !
Thank you
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