How do i get rid of Formamide from my dsDNA? Edit
I used hybridization buffer mix containing 70% formamide to renature my single stranded DNA. The hybridization was successfull as Qubit measured dsDNA. I now wish to get rid of formamide from my dsDNA since the viscosity does not allow me to proceed with shearing (Covaris) DNA for seq. Any suggestions?
Note the following methods did not work:
1. I have tried using precipitation method with isopropanol
2. I used both YM30 and YM50 purification columns to get rid of formamide
3. I used magnetic beads to pull out my DNA
4. I used a combination of 1/3 , 1/2, 2/3
5. I used 0.4 SSC containing Tween to wash away the formamide in 1:1 and 2:1 volumes following by 4 MQ washing steps - it only reduced the viscosity but did not get rid of it i.e. final product was still viscous.*
6. A combination of 1/5/2
Nothing above gave me my desired product.*
Appreciate any advise or feedback. Thanks.
I used hybridization buffer mix containing 70% formamide to renature my single stranded DNA. The hybridization was successfull as Qubit measured dsDNA. I now wish to get rid of formamide from my dsDNA since the viscosity does not allow me to proceed with shearing (Covaris) DNA for seq. Any suggestions?
Note the following methods did not work:
1. I have tried using precipitation method with isopropanol
2. I used both YM30 and YM50 purification columns to get rid of formamide
3. I used magnetic beads to pull out my DNA
4. I used a combination of 1/3 , 1/2, 2/3
5. I used 0.4 SSC containing Tween to wash away the formamide in 1:1 and 2:1 volumes following by 4 MQ washing steps - it only reduced the viscosity but did not get rid of it i.e. final product was still viscous.*
6. A combination of 1/5/2
Nothing above gave me my desired product.*
Appreciate any advise or feedback. Thanks.
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