Dear All,
Has anyone done multiplex PCR for targeted sequencing on MiSeq?
What software have you used to design the primers?
If My Illumina sequencing adapters are part of the target region primers, wouldn't that overhand sequence interfere with my PCR, especially in a multiplex reaction?
I don't know why I feel so nervous having a primer overhang.
Please, share your experience, thoughts, etc.
Thank you!
Dessi
Has anyone done multiplex PCR for targeted sequencing on MiSeq?
What software have you used to design the primers?
If My Illumina sequencing adapters are part of the target region primers, wouldn't that overhand sequence interfere with my PCR, especially in a multiplex reaction?
I don't know why I feel so nervous having a primer overhang.
Please, share your experience, thoughts, etc.
Thank you!
Dessi
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