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Old 07-04-2017, 06:05 AM   #1
Arthur45
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Default maximum size of fragment as input for BluePippin?

Hello all,

I ran a library on the BluePippin using a 2% agarose cassette (V1 marker), with the hope to size select from 150 to 450bp.

The input library had two peaks detected by TapeStation: a short peak around 200bp (what we want to sequence) and above 1000bp (what we wanted to remove with the BluePippin).

However the traces after BluePippin show that the large fragment peak is still here. Does anyone know what happened here?

I am wondering whether the 2% cassette only allows fragments of a certain size. Do you know if there is such a thing as maximum size of fragment as input for BluePippin?

Cheers,

Arthur
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Old 08-04-2017, 08:42 AM   #2
Arthur45
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Just a follow-up, the tech from Sage told me that my samples has too much salt in them, so that the BluePippin could not separate properly the DNA strands. Good to know!
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