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Old 08-08-2017, 03:00 AM   #1
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Location: Italy

Join Date: Aug 2017
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Default BLAST possibly returning different results

Hi to everyone,
this is my very first post and first of all I'd like to thanks Seqanswers for the possibility offered.

I've submitted the following sequence to BLASTN:

among the results I received also a single match to the following organism:
Clostridium botulinum Ba4 str. 657 GenBank CP001083.1

so I tried and run BLASTN again specifying the organism in the Organism field of BLASTN.

Surprisingly I did obtain 4 matches now (apart from plasmid). They are highlighted in black as poor results but why did BLASTN provide them now only?

Thanks for any help

Last edited by Jona; 08-08-2017 at 03:04 AM.
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