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Old 08-08-2017, 12:44 AM   #1
odyssey
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Location: Russia

Join Date: Feb 2017
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Question UniFrac analysis for Sanger and 454 pyroseq data

Hi, all!
I have a dataset from Sanger (12 seqs ~350-500bp) and 454 sequencing (32000 seqs (140 OTUs) ~500bp).

And here is a question:
It's possible to compare OTUs seqs from 454 with Sanger by weighted\unweighted UniFrac analysis?

And one more question: It's correctly to do this if I have so different size of datasets - 12 seqs vs. 140 OTUs? Could I say that my 12 Sanger seqs are equivalent to OTUs in metagenome?

Thanks,
Kind regards!
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