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Old 08-24-2013, 08:51 AM   #1
bios90
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Default Any use for a 20 base sequencer?

Any use for it?

Last edited by bios90; 02-24-2014 at 08:04 PM. Reason: Could be proprietary information
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Old 08-24-2013, 01:11 PM   #2
SNPsaurus
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Sequencing of medically-relevant SNPs. Each target is one or a few nucleotides, so a quick PCR and read-out would have a use. Maybe still not competitive if other platforms can do more at the same cost and speed.

Expression analysis... just need a short tag of the transcript. miRNAs are short anyway. Local assembly could convert short reads into longer pseudoreads (see RAD PE assembly or Moleculo).
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Old 08-24-2013, 02:33 PM   #3
austinso
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Probably not the best idea to publicize your PhD before it is complete...you don't want to get scooped.
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Old 08-27-2013, 08:40 AM   #4
krobison
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A key question to ask of your technology is how it will perform in the presence of a non-clonal DNA sample. Simplest case is an essentially 50:50 mixture (heterozygote), but for other applications could it detect either an infrequent allele or many mixed alleles.
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Old 08-27-2013, 09:03 AM   #5
SNPsaurus
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Yes, you could screen cell-free blood DNA for tumor alleles (at less than 1% frequency) if you had an excellent error rate and reduced the time for turning sample -> data.
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Old 09-12-2013, 09:45 PM   #6
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And more importantly, how is this better than established array technology. FET sensing of hybridization has been published time and again over the past 10 years.
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