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Old 05-01-2014, 07:29 PM   #1
spikey_hedgehog
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Location: Australia

Join Date: Feb 2012
Posts: 5
Default digital PCR & ChIP-seq (internal control?)

Hi guys,

I am very new to making my own libraries and I am interested in using digital PCR to QC my ChIP-seq libraries. So I would have the universal primers spanning the adaptors and run the diluted libraries on the chip.

Is it necessary to have an internal control for this? Other people have used RNAse P but that's only if I know RNAse P will be enriched in my library..right?

How do people choose their internal control (if they have one)?

Thanks heaps! Look forward to your answers!
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