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Old 06-28-2012, 06:46 PM   #1
Ddhamburger
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Default RNAseq by PacBio

Hi, all
I was wondering if PacBio is an idea platform for RNAseq due to its long read length.
Or Is it good to combine PacBio data with illumina's?
Thanks
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Old 06-28-2012, 08:15 PM   #2
ECO
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For looking at isoforms of a small set of genes, almost definitely, pending you can make ds cDNA long enough.

Almost certainly not enough tput to be useful for whole transcriptome.
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Old 06-29-2012, 03:17 AM   #3
GenoMax
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PacBio is also going to be more expensive overall. Error rate with PacBio will cause problems unless you have other supplemental data (illumina, Solid etc) or a good reference sequence.
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