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Old 05-16-2012, 09:20 PM   #1
lcarey
Junior Member
 
Location: Weizmann, Israel

Join Date: May 2012
Posts: 4
Lightbulb Wouldn't an RNase+ reverse transcriptase give better library representation?

Hi All,
I will be building a library for mRNA Seq where I want to count transcripts (measure gene expression). Wouldn't using a reverse transcriptase that has RNaseH activity be better for this because it will prevent priming from the same molecule twice, and therefore the cDNA pool will more precisely mirror the mRNA pool?
Why does everyone use rnaseh- RT?

-Lucas
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library construction, mrna, reverse transcriptase, reverse transcription

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