Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Wouldn't an RNase+ reverse transcriptase give better library representation? lcarey Sample Prep / Library Generation 0 05-16-2012 09:20 PM
PubMed: Ultra-deep sequencing of HIV-1 reverse transcriptase before start of an NNRTI Newsbot! Literature Watch 0 04-20-2012 02:00 AM
PubMed: Development of a novel real-time reverse-transcriptase PCR method for the det Newsbot! Literature Watch 0 05-12-2009 05:00 AM

Thread Tools
Old 03-27-2015, 06:30 AM   #1
Junior Member
Location: Guangdong, China

Join Date: Mar 2015
Posts: 5
Default Which nucleotide does the reverse transcriptase stop at in SHAPE reaction?

Hi, everyone here!
Now I confuse about the exact position which reverse transcriptase stop at in SHAPE (Selective 2′-hydroxyl acylation analyzed by primer extension) reaction. Is the nucleotide modified by MDS (may be some times 1M7 or NAI-N3) or the nucleotide before the modified one? Is the last base of RT product corresponding to the modified nucleotide (5-3)? If you are interested in the topic and know the answer, please tell me.
Many thanks!
huiyecao062 is offline   Reply With Quote

reverse transcriptase, rna, shape

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:45 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO