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Old 03-27-2015, 06:30 AM   #1
huiyecao062
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Location: Guangdong, China

Join Date: Mar 2015
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Default Which nucleotide does the reverse transcriptase stop at in SHAPE reaction?

Hi, everyone here!
Now I confuse about the exact position which reverse transcriptase stop at in SHAPE (Selective 2′-hydroxyl acylation analyzed by primer extension) reaction. Is the nucleotide modified by MDS (may be some times 1M7 or NAI-N3) or the nucleotide before the modified one? Is the last base of RT product corresponding to the modified nucleotide (5-3)? If you are interested in the topic and know the answer, please tell me.
Many thanks!
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