Hi all,
I am looking into methods that would be able to resequence ~0.5 - 1 Mb region which contains repetitive/GC rich regions. I would like to do this to see if there is a large structural variation going on in here (large inversion). I only have a few samples that I would like to do this on (at most 4) so it does not have to be scaled, but still cost effective and not requiring massive amounts of DNA.
Does any one have any recommendation/experience on how to go about this ? In particular, can anyone comment on mate pair sequencing vs 454 pyrosequencing, as well as hybrid capture and long range for enrichment ?
Any ideas greatly appreciated , thanks !
I am looking into methods that would be able to resequence ~0.5 - 1 Mb region which contains repetitive/GC rich regions. I would like to do this to see if there is a large structural variation going on in here (large inversion). I only have a few samples that I would like to do this on (at most 4) so it does not have to be scaled, but still cost effective and not requiring massive amounts of DNA.
Does any one have any recommendation/experience on how to go about this ? In particular, can anyone comment on mate pair sequencing vs 454 pyrosequencing, as well as hybrid capture and long range for enrichment ?
Any ideas greatly appreciated , thanks !
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