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Old 07-15-2011, 04:55 AM   #1
leesi
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Location: London

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Default Experience of Illumina TruSeq Exome coverage?

Hi,
I'm wondering what coverage others are seeing with the Illumina TruSeq Exome, specifically % bases with either 10, 15 or 20x coverage. We're running 6 samples in a pool, across 2 lanes - any idea what we should expect? An average measure isn't as helpful, since I'm more keen to quantify what we're missing.
Cheers!
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Old 07-15-2011, 08:31 AM   #2
NextGenSeq
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Roughly 2/3rds are greater than 10X coverage when a single exome is run in a HiSeq lane. This wasn't a v3 run though.
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Old 03-06-2013, 12:45 AM   #3
chongm
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Default Truseq 62 Mb exome enrichment

With the 62 Mb exome enrichment, on a hiseq lane we 6-plexed and got ~70%PCTbases > 20x
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