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Thread Tools |
02-01-2012, 07:20 AM
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#1 |
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Junior Member
Location: Frankfurt Join Date: Feb 2012
Posts: 2
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comprehensiveness of sequence coverage in miRNA-, ChIP-, RNA-seq?
My problem: How figure out if the number of reads in sequencing I have is high enough to 1. cover ‘all’ target miRNAs/binding sites/mRNAs and 2. have high enough coverage to allow for stable quantification of target sequences.
Any help? Thank you :-) |
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02-01-2012, 11:55 PM
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#2 |
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Senior Member
Location: Munich Join Date: Jan 2009
Posts: 138
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for chipseq:
P. V. Kharchenko, M. Y. Tolstorukov, and P. J. Park. Design and analysis of chIP experiments for DNA–binding proteins. Nature Biotechnology, 26:1351–1359, 2008. |
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02-02-2012, 12:07 AM
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#3 |
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Junior Member
Location: Frankfurt Join Date: Feb 2012
Posts: 2
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Thanx a lot, this should work also for miRNA- and mRNA-seq.
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