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Old 09-09-2014, 05:15 AM   #1
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 833
Default 2-hour run on Oxford Nanopore MinION

I am participating in the MinION access programme, and noticed that Nick Loman recorded a Nanopore run last night, using the most recent v7.3 flow cells. You can see the final read length histogram here:

https://www.youtube.com/watch?v=lFmBO2CeMTg#t=2h5m

[or watch from the start to see the entire 2h run]

This run produced a little under one read per second, with average read length about 7kbp, maximum sequence length of about 68kbp, producing about 45 Mbp over the course of the run. These numbers are estimated from the event data produced by the software, and the number of mappable bases will be less than this.

To reduce confusion slightly, the histogram shown in the MinKNOW program here is adjusted so that the bin height displays the summed length of sequences in each bin, rather than the number of sequences. As an example of interpretation for this run, the most base pairs were generated from sequences that were about 20kbp long.
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