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Old 02-07-2015, 01:58 PM   #21
floem7
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Location: Poland, Warsaw

Join Date: Jan 2013
Posts: 10
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Recently we have presentation of Fragment Analyser.

It's big advantage is lack of pricey chips, one just have to buy some chemicals. Another one plus is great scalability.

Although, as it depends on bottle with appropriate gel you have to replace it if you plan to reslove other molecules (DNA vs RNA). So it's best if you have many samples of one type (DNA/RNA) to measure.
During presentation we resolved marker made from digested pUC, I was quite suprised to see that fragment sizing was a little different from provided by pUC marker flyer (but relative band sizes were ok). For each measurement one have to use internal marker (small fragment and big fragment) and measurement is somehow compromised if molecules in sample have similar size (this two-band marker and sample is resolved in one capillary).

It, also can't resolve proteins.

Nevertheless I regret I can't afford it.
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