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Old 02-23-2015, 02:03 AM   #1
RajaRaghu
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Location: Canada

Join Date: Aug 2012
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Default HiSeq Vs MiSeq

Hi all,
We constructed a library, using 140-160bp cut fragment as recommended by illumina. We did a 1X50bp run. We compared it with HiSeq data we obtained earlier.
1. In all cases, the HiSeq is producing a higher number of 21nt than 24nt but the reverse is true for the MiSeq run. Is there any technical reason for this bias?
2. We observe a peak at 32-33nt in the MiSeq. what it represents? Any suggestions?

Thanks for your reply.
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Old 02-23-2015, 06:18 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

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Default

I can't think of a reason to think the instruments are responsible for this. Seems far more likely that library construction for the previous HiSeq data differed from that for your MiSeq run.

--
Phillip
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Old 03-03-2015, 01:24 AM   #3
RajaRaghu
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Thumbs up

Thanks Phillip.
We also came to the same conclusion.
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