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Old 11-20-2017, 03:49 AM   #1
emmannaemeka
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Location: Nigeria

Join Date: Jan 2017
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Default How to produce consensus genome

I have two sequences I wish to assemble to give a consensus genome. I am using Velvet assembler

would this commandline be OK

./velveth conref_out 35 -fastq.gz -shortPaired -separate /Users/emmannaemeka/Desktop/QTL_ANALYSIS/Trimmomatic-0.36/EN28FP.fq.gz //Users/emmannaemeka/Desktop/QTL_ANALYSIS/Trimmomatic-0.36/EN28RP.fq.gz /Users/emmannaemeka/Desktop/QTL_ANALYSIS/Trimmomatic-0.36/KN99aFP.fq.gz /Users/emmannaemeka/Desktop/QTL_ANALYSIS/Trimmomatic-0.36/KN99aRP.fq.gz

were FP= forward paired RP= Reverse paired EN28= Sequence 1 KN99a= Sequence 2

Thanks
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Old 11-20-2017, 04:15 AM   #2
GenoMax
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Default

For reference cross-posted: https://www.biostars.org/p/284783/
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