Hi everyone,
I am new to bioinformatics and I have been having trouble with exome data. I've practised using standalone BLAST+ and I've understood how to create databases with my own sequences, BLAST another set of data, return values using outfmt, etc. However, I still cannot for the life of me understand how to use exome data (sequences contain gaps) and blast them when I have a set of CDS genes that I want to find. i.e. I have exome data for 10 fish species, and I want to find their genes associated with aquaporins (sequences I have using zebrafish reference).
I've tried everything but I just get errors, not can I figure out how to show my query sequences in the output. (using Windows 10 if that matters).
I am new to bioinformatics and I have been having trouble with exome data. I've practised using standalone BLAST+ and I've understood how to create databases with my own sequences, BLAST another set of data, return values using outfmt, etc. However, I still cannot for the life of me understand how to use exome data (sequences contain gaps) and blast them when I have a set of CDS genes that I want to find. i.e. I have exome data for 10 fish species, and I want to find their genes associated with aquaporins (sequences I have using zebrafish reference).
I've tried everything but I just get errors, not can I figure out how to show my query sequences in the output. (using Windows 10 if that matters).
Comment