Hi all,
My application requires short cDNA fragments (up to 500 bp) with a defined 5' tag. I don't have the option to fragment RNA before reverse transcription using Mg and heat or the option to fragment afterwards via tagmentation or sonication. Does anyone have any ideas for the best way to accomplish this?
My application requires short cDNA fragments (up to 500 bp) with a defined 5' tag. I don't have the option to fragment RNA before reverse transcription using Mg and heat or the option to fragment afterwards via tagmentation or sonication. Does anyone have any ideas for the best way to accomplish this?
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