SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
What are the default settings in bwa? genomicist Bioinformatics 0 02-06-2012 01:52 AM
[Mosaik] - parameter settings Cirno Bioinformatics 0 11-05-2011 03:57 PM
GATK indel settings help please inou13 Bioinformatics 2 10-25-2011 08:09 AM
samtools mpileup parameter settings xxy39 Bioinformatics 1 05-28-2011 05:40 PM
soapaligner - settings -M/-v tilsith Bioinformatics 0 01-04-2011 06:18 AM

Reply
 
Thread Tools
Old 05-09-2013, 04:59 AM   #1
hawaii454-0
Member
 
Location: UK

Join Date: Dec 2010
Posts: 23
Default qPCR settings

Hello,
What threshold do you set for a qPCR run for quantifying libraries? We use KAPA Library Quantification Kits on an Eco instrument. We currently use 0.06 but are concerned it may be too low. Should there be different settings for the type of sample, for example RAD libraries or TruSeq libraries?

Any advice you could give would be much appreciated.

Thanks!
hawaii454-0 is offline   Reply With Quote
Reply

Tags
kapa, qpcr, quantitation, truseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:10 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO