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Old 05-09-2013, 04:59 AM   #1
Location: UK

Join Date: Dec 2010
Posts: 23
Default qPCR settings

What threshold do you set for a qPCR run for quantifying libraries? We use KAPA Library Quantification Kits on an Eco instrument. We currently use 0.06 but are concerned it may be too low. Should there be different settings for the type of sample, for example RAD libraries or TruSeq libraries?

Any advice you could give would be much appreciated.

hawaii454-0 is offline   Reply With Quote

kapa, qpcr, quantitation, truseq

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