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  • Total gene discovered/detected is less than gene in reference genome

    Dear All,

    I did a RNA-seq on my bacteria (Salmonella) samples for a differential expression study. I used the cufflinks pipeline for this study.

    When I checked the results of cuffdiff it showed 3752 genes but when I checked the Salmonella reference gene list it shows a total of 4949 genes. How is there such a massive difference in the gene numbers?

    As I understand some of the genes may not be detected due to inactivation or are hypothetical genes. However this is a not satisfactory answer for me and I would like to know if anyone has an explanation for this. I may be looking at the wrong keywords so if possible can someone please point me in the correct direction for me to read up on this?

    Thank you.

  • #2
    Hi,
    I think it's a good result ! I'm not specialist in bacteria but if i remember rigth there is a big diversity in population and the genomic dynamic is intensive ( i speak about bacteria in general i don t know if this rules match for Salmonella). But if I’m right if you add the genomic diversity and the temporal expression plus extraction bias, at the end you have 75% of the genes describes , is not bad , don't you think ?
    Depends at the end what are you looking for ?
    An other point is i don't know witch databases you use but you have to be care about it because you have 2 types of information :
    -Gene describe (protein expressed function etc )
    -Gene predicted , based on functional domain who are described on other protein and genomic structure/pattern

    Comment


    • #3
      Hi Tristan,

      Thanks for the input. I totally did not take into consideration the RNA extraction bias and the sequencing bias as well.

      Thanks for your help.

      I was using the RefSeq complete genome reference from NCBI as my reference.

      Comment

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