SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Primer dimer problem cybeline Illumina/Solexa 76 11-20-2018 12:00 PM
primer3 krunal Bioinformatics 3 05-26-2014 05:08 AM
primer dimer problem in PCR using Illumina protocol Neiltje Illumina/Solexa 1 05-16-2014 04:11 PM
Primer dimer in ChIP-seq library lavagirl Sample Prep / Library Generation 7 06-07-2013 05:52 AM
primer dimer CCBIO 454 Pyrosequencing 5 03-18-2013 10:31 AM

Reply
 
Thread Tools
Old 10-23-2014, 06:29 AM   #1
ingridejt
Junior Member
 
Location: Pennsylvania

Join Date: Nov 2013
Posts: 1
Default Primer3 primer dimer score

I have used Primer3 tool within Geneious to design a large number of primers. There is one parameter that I would like some help in interpreting. The primer dimer score, which ranges from 0 to a large number of some kind, is somewhat confusing. How is this calculated? What is considered a “good” number for primer dimers not to form? Obviously zero is ideal, but when I look at my dataset, It ranges up to 30 or so, with everything in between. The online help information gives examples of 6 or 7. I would appreciate any laboratory experience advice on what would be a good threshold to avoid. I am developing primers for Fluidigm/Illumina. Thanks!
ingridejt is offline   Reply With Quote
Reply

Tags
fluidigm, primer-dimer, primer3, score

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:26 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO