Two of my libraries failed on HiSeq, due to focussing error of the instrument. The cluster densities were approx. 800-900 K/mm2 but %PF was low (50-60%). Just to be sure that my libraries are not bad, I want to run them on MiSeq.
I do not have any fresh library remaining with me. Can I use the denatured 20PM ones that I stored after setting the HiSeq run?
Has anyone tried something like this? It's been 10 days since the library has been diluted and they were stored at -20C.
I do not have any fresh library remaining with me. Can I use the denatured 20PM ones that I stored after setting the HiSeq run?
Has anyone tried something like this? It's been 10 days since the library has been diluted and they were stored at -20C.
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