Can anyone explain how to design RNA bait for whole genome sequencing?
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I cannot work out why you would need RNA bait for whole genome sequencing. For RNA bait design for targeted sequencing following links might be useful:
http://www.mycroarray.com/mybaits/mybaits-custom.html
https://earray.chem.agilent.com/suredesign/
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It can apparently be helpful for things like ancient DNA extractions where the percentage of exogenous DNA is high.
This is the paper I know of, and they have instructions of making the baits yourself (I've never tried it):
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Following is a link to aDNA sequencing using a predesigned capture kit:
http://www.mycroarray.com/mybaits/mybaits-WGE.html
or you have to follow that paper's method to produce your own baits.
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