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Old 01-20-2016, 12:17 PM   #1
candicechu
Junior Member
 
Location: College Station

Join Date: Nov 2015
Posts: 3
Default Concentrating method for TruSeq lib prep in microRNA sequencing

Hi all,

We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

We use column-based isolation methods (e.g. mirVana PARIS) and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples.

I was wondering how can we concentrate our microRNA samples? Does anybody have any experience with the vacuum method?

Any suggestions are appreciated. Thanks!
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Old 01-20-2016, 04:40 PM   #2
nucacidhunter
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Location: Melbourne

Join Date: Jan 2013
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SpeedyVac volume reduction set at RT is a good method for your samples as they have been eluded with water and there is not any risk of salt accumulation or loss of sample.
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concentrate, microrna, microrna sequencing, truseq

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