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Old 12-23-2013, 06:55 AM   #1
Samarpana
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Location: INDIA

Join Date: Dec 2013
Posts: 16
Default Illumina Truseq PCR enrichment pre-pooling issue

Hi
I am currently using Truseq kits. Just finished with sample prep. On gel, I could distinctively see one bright band but after gel extraction followed by PCR amplification, Bioanalyzer is showing 2 peaks - one between 200-500bp(desired) and other around 9bp-1kb!! The intensity of both are similar.
Could it be a problem with PCR kit?
I am really confused. Please suggest possible reason for this and a solution, if any.
P.S. I diluted my DNA sample with crosslinked MQ for Bioanalyzer reading?
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illumina, pcr cleanup, truseq

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