Simulation of a small RNA-seq experiment

[RikyJKD]

Hi, I would like to know if it is possible to simulate a small RNA-seq dataset because I found tools only for RNA seq.
Thank you.

[Brian Bushnell]

Perhaps you could clarify your goal?

[RikyJKD]

I would like to have a synthetic small RNA-seq for validation purposes.

[Brian Bushnell]

That's pretty vague. Since you are not stating any specific goals or metrics, I recommend you download some real small-RNA data from somewhere (such as SRA) and use that for validation. If you are not trying to measure anything in particular, real data is always better than synthetic data.

[GenoMax]

Are you looking for small (as in amount of reads/data) or small as in small RNA-seq data?

[RikyJKD]

Small as in small RNA-seq data, like a real small rna sequencing for miRNAs.

[GenoMax]

I don't know of a simulation program that can generate smallRNA seq data. Can you use a real dataset from here?

[RikyJKD]

Thank you.