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Old 01-24-2011, 09:46 AM   #1
OptimusBrien
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Location: Dublin

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Default Amplification of ChIP'd DNA before library prep

Hey Guys,
Does anyone have first hand experience of amplifying ChIP'd DNA before preparing an Illumina library? For reasons I will not go into hear I need to amplify my material. I've seen suggestions of a protocol involving amplification followed by concatenation of the amplified fragments and subsequent fragmentation of the concatenated DNA. But have had difficulty finding somebody with first hand experience whose brain I can pick If anyone has any experience I'd really appreciate some help
Cheers
Optimus
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Old 07-12-2012, 12:26 PM   #2
mira636
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Default Also interested in this topic

Hi,

I'm looking into amplifying my Chip'd DNA as well before library preparation. I currently have Chip'd DNA in the pg range and I need to amplify up to 20ng. What protocol did you end up doing?

Thanks in advance!
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