Hi,
We've been running GAII with 2x108 and a 64nt spacer (280nt insert) without problems for a while. We've also been running long insert mate pair reads on 454 with a few more problems (library over-reading, missing adapter sequences, higher costs etc).
What I'd like to do is to try shifting over to 5kbase insert GAII mate pair runs. But I'm going blind into this in terms of any new sequencing or analysis issues we may face. Could anyone provide first hand advice on whether the mate-pair procedure on GAII runs as smoothly as a normal short insert PE run?
Thanks!
We've been running GAII with 2x108 and a 64nt spacer (280nt insert) without problems for a while. We've also been running long insert mate pair reads on 454 with a few more problems (library over-reading, missing adapter sequences, higher costs etc).
What I'd like to do is to try shifting over to 5kbase insert GAII mate pair runs. But I'm going blind into this in terms of any new sequencing or analysis issues we may face. Could anyone provide first hand advice on whether the mate-pair procedure on GAII runs as smoothly as a normal short insert PE run?
Thanks!
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