Hi guys, I'm constructing a PE library by using the Nextera DNA library Prep Kit (Illumina compatible). However, when I cut down the 300-350bp, 450-500bp, 600-650bp region separately after 2.0% agarose gel electrophoresis, there comes double peaks instead of a single narrow peak (pictures). I'm sure there's no problem during gel selection, but obviously, this cannot meet the requirement of a PE library. Is there anyone who has some ideas on this?
Start with 150ng gDNA, and after 10 cycles of PCR:
gel selection 300-350bp:
gel selection 450-500bp:
gel selection 600-650bp:
Start with 150ng gDNA, and after 10 cycles of PCR:
gel selection 300-350bp:
gel selection 450-500bp:
gel selection 600-650bp:
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